Antifungal Activity of Rye Binding Manner of Class I JSBA ( Secale cereale ) Seed Chitinases : the Different and Class II Chitinases to the Fungal Cell VValls
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چکیده
cell walls prepured from its mycelia. RSC-a inhibited . more strong}y the re-extension of the hyphae, containing mainly mature cells, than RSC-c did. Upon incubation of the fungus vvith fluorescent chitinases, FITC}abeled RSC-a was found to be located in the hyphal tips, lateral walls, and septa, whi]e FITC-labeled RSC-c was only in the hyphal tip. RSC-a had a greater aMnity for the cell walls than RSC-c. RSC-a liberated a larger amount of reducing sugar from the cell walls than RSCc did. These reslllts inferred that RSC-a first binds to the
منابع مشابه
Localization , Accumulation , and in Rye ( Secale cereale ) Seed AntifungalActivity of Chitinases
In order to understand a physiologi¢ al role of chitinases in rye, the localization and accumulatien of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by imm"nochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), whicb is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot a...
متن کاملChitinase genes responsive to cold encode antifreeze proteins in winter cereals.
Antifreeze proteins similar to two different chitinases accumulate during cold acclimation in winter rye (Secale cereale). To determine whether these cold-responsive chitinases require post-translational modification to bind to ice, cDNAs coding for two different full-length chitinases were isolated from a cDNA library produced from cold-acclimated winter rye leaves. CHT9 is a 1,193-bp clone th...
متن کاملMolecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c.
We cloned a complete cDNA encoding rye seed chitinase-c, designated RSC-c, by rapid amplification of cDNA end and PCR procedures. The cDNA of RSC-c consists of 1,018 nucleotides and includes an open reading frame encoding a polypeptide of 266 amino acid residues. A recombinant RSC-c was produced by expression in Escherichia coli Origami(DE3) and purified. rRSC-c had almost the same chitinase ac...
متن کاملLocalization , Accumulation , and in Rye ( Secale
In order to understand a physiologi¢ al role of chitinases in rye, the localization and accumulatien of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by imm"nochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), whicb is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot a...
متن کاملThe complete amino acid sequence of chitinase-a from the seeds of rye (Secale cereal).
The complete amino acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleaved with cyanogen bromide and the resulting three fragments, CB1, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CB1 were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin of reduced S-carboxymethylated ...
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